Global analysis of gene expression profiles and gout symptoms in goslings infected with goose astrovirus.
Vet Microbiol
; 279: 109677, 2023 Apr.
Article
in En
| MEDLINE
| ID: mdl-36764218
ABSTRACT
While blocking inflammation is an effective way to ease the symptoms of gout disease in humans, the treatment and prevention of gout in goslings infected with goose astrovirus (GAstV), a recently emergent condition, remain unclear. In this study, we investigated the reprogramming of the host genes as a result of GAstV infection by combining analysis of the global transcriptome and metabolic network pathways in the kidneys of goslings infected with GAstV. We showed that as GAstV replication increased in vivo, the regulation of key enzymes in the host metabolism progressively increased, flowing metabolites into the purine/pyrimidine biosynthesis pathways. Furthermore, we found that GAstV 1) inhibits the host oxidation-reduction response by inhibiting the expression of the catalase gene; 2) activates the Toll-like receptor 2 pathway to enhance the immune inflammatory response; and 3) activates the key enzyme in lactic acid synthesis to produce lactate accumulation which inhibits the host's antiviral response, so as to facilitate the replication of the virus itself. This study provided the first insight into the overall metabolic requirements of GAstV for replication in vivo by combining transcriptome with metabolic network pathway information.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Poultry Diseases
/
Astroviridae Infections
/
Avastrovirus
/
Gout
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Vet Microbiol
Year:
2023
Document type:
Article