Hepatocyte growth factor ameliorates dextran sodium sulfateinduced colitis in a mouse model by altering the phenotype of intestinal macrophages.
Mol Med Rep
; 27(3)2023 Mar.
Article
in En
| MEDLINE
| ID: mdl-36799161
ABSTRACT
Hepatocyte growth factor (HGF) serves key roles in cell motility, proliferation and immunoregulatory functions. However, the effect of HGF on macrophages is unclear. The present study aimed to elucidate the effect of HGF on the phenotypic alterations of intestinal lamina propria mononuclear cells (LPMCs). Colitis was induced in a mouse model using dextran sodium sulfate (DSS). Subsequently, LPMCs were isolated from the mice with chronic colitis and the expression levels of cytokineencoding genes in the LPMCs were determined. CD11bpositive macrophages isolated from LPMCs were cultured with HGF, and alterations in the levels of M1 or M2 markers were evaluated by reverse transcriptionquantitative polymerase chain reaction (RTqPCR) and flow cytometry. In addition, the cytokine levels were assessed using RTqPCR and ELISA. HGF shifted the phenotype of macrophages from M1 to M2like, as determined by increased mRNA expression levels of arginase1, CD206 and IL10, and reduced mRNA expression levels of CD86 and IL6 in mice with DSSinduced colitis. Moreover, HGF could ameliorate DSSinduced colitis owing to its immunosuppressive effect on immune cells. These findings indicated that HGF treatment may not only promote the regeneration of epithelial cells but also lead to tissue repair by phenotypic alteration of M1 macrophages to M2like macrophages.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hepatocyte Growth Factor
/
Colitis
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Mol Med Rep
Year:
2023
Document type:
Article
Affiliation country:
Japan