Distinct stage-specific transcriptional states of B cells derived from human tonsillar tissue.
JCI Insight
; 8(7)2023 04 10.
Article
in En
| MEDLINE
| ID: mdl-36809296
ABSTRACT
B cells within secondary lymphoid tissues encompass a diversity of activation states and multiple maturation processes that reflect antigen recognition and transition through the germinal center (GC) reaction, in which mature B cells differentiate into memory and antibody-secreting cells (ASCs). Here, utilizing single-cell RNA-seq, we identify a range of distinct activation and maturation states of tonsil-derived B cells. In particular, we identify what we believe is a previously uncharacterized CCL4/CCL3 chemokine-expressing B cell population with an expression pattern consistent with B cell receptor/CD40 activation. Furthermore, we present a computational method that leverages regulatory network inference and pseudotemporal modeling to identify upstream transcription factor modulation along a GC-to-ASC axis of transcriptional maturation. Our data set provides valuable insight into diverse B cell functional profiles and will be a useful resource for further studies into the B cell immune compartment.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Palatine Tonsil
/
B-Lymphocytes
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
JCI Insight
Year:
2023
Document type:
Article