Fast and sensitive GCaMP calcium indicators for neuronal imaging.
J Physiol
; 602(8): 1595-1604, 2024 Apr.
Article
in En
| MEDLINE
| ID: mdl-36811153
ABSTRACT
We review the principles of development and deployment of genetically encoded calcium indicators (GECIs) for the detection of neural activity. Our focus is on the popular GCaMP family of green GECIs, culminating in the recent release of the jGCaMP8 sensors, with dramatically improved kinetics relative to previous generations. We summarize the properties of GECIs in multiple colour channels (blue, cyan, green, yellow, red, far-red) and highlight areas for further improvement. With their low-millisecond rise-times, the jGCaMP8 indicators allow new classes of experiments following neural activity in time frames approaching the underlying computations.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Physiol
Year:
2024
Document type:
Article
Affiliation country:
United States