Generation of an RNA aptamer against LipL32 of Leptospira isolated by Tripartite-hybrid SELEX coupled with in-house Python-aided unbiased data sorting.

Shien Yeoh, Tzi; Yusof Hazrina, Hamdani; Bukari, Bakhtiar A; Tang, Thean-Hock; Citartan, Marimuthu

Shien Yeoh, Tzi; Yusof Hazrina, Hamdani; Bukari, Bakhtiar A; Tang, Thean-Hock; Citartan, Marimuthu.

Affiliation

Shien Yeoh T; Department of Biomedical Science, Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia.
Yusof Hazrina H; Department of Biomedical Science, Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia.
Bukari BA; School of Medicine, Deakin University, 3216 Geelong, Victoria, Australia.
Tang TH; Department of Biomedical Science, Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia.
Citartan M; Department of Biomedical Science, Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia. Electronic address: citartan@usm.my.

Bioorg Med Chem ; 81: 117186, 2023 03 01.

Article in En | MEDLINE | ID: mdl-36812779

Subject(s)

Aptamers, Nucleotide; Boidae; Leptospira; Leptospirosis; Animals; Humans; Leptospira/genetics; Boidae/metabolism; Lipoproteins/genetics; Leptospirosis/diagnosis; Leptospirosis/metabolism; Bacterial Outer Membrane Proteins/metabolism

Key words

LipL32; RNA aptamer; Tripartite-hybrid SELEX

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Boidae / Aptamers, Nucleotide / Leptospira / Leptospirosis Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Bioorg Med Chem Journal subject: BIOQUIMICA / QUIMICA Year: 2023 Document type: Article Affiliation country: Malaysia Country of publication: United kingdom

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