Effects of Two Calcium Silicate Cements on Transforming Growth Factor-ß1 Secretion from Human Dental Pulp Stem Cells.
Iran Endod J
; 13(4): 522-527, 2018.
Article
in En
| MEDLINE
| ID: mdl-36883037
ABSTRACT
Introduction:
The aims of this in vitro study were to evaluate the effects of two calcium silicate based cements, Calcum-enriched Mixture (CEM) and Biodentine on proliferation of human dental pulp stem cells (hDPSCs) and the effects of proposed cements on the secretion of Transforming Growth Factor ß1 (TGF-ß1). Methods and materials The cell cultures of human Dental Pulp Stem Cells (hDPSCs) at passage 3-5 were treated with various dilutions (1/1, 1/2, 1/4, 1/8, 1/16, and 1/32) of CEM and Biodentine extracts to assess the cell proliferation using 3-(4, 5-dimethylthiazol-2-Y1)-2, 5-diphenyltetrazolium brovide (MTT) assay after 48 and 72 h. The amount of TGF-ß 1 secretion were estimated after 72 h using an enzyme-linked immunosorbent assay. Data were analyzed using the one-way analysis of variance (ANOVA) followed by the Dunnett's test at the level of significance set at 0.05.Result:
CEM showed the highest rates of cell proliferation compared to Biodentine after 72 h (P<0.05). A greater amount of TGF-ß1 was secreted by hDPSCs treated with Biodentine compared to CEM (P<0.05). These differences were statistically significant (P<0.05).Conclusion:
In this in vitro study hDPSCs showed more proliferation capacity with CEM rather than Biodentine and TGF-ß1 secretion rate in Biodentine was higher.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Iran Endod J
Year:
2018
Document type:
Article
Affiliation country:
Iran