The human cumulus cell transcriptome provides poor predictive value for embryo transfer outcome.
Reprod Biomed Online
; 46(5): 783-791, 2023 05.
Article
in En
| MEDLINE
| ID: mdl-36922313
ABSTRACT
RESEARCH QUESTION Is the transcriptome of cumulus cells a good predictor of the embryo's developmental competence? DESIGN:
Cumulus cells were collected from donor oocytes and their transcriptome was analysed by RNA sequencing analysis at >30â¯×â¯106 reads in samples grouped according to the developmental potential of their enclosed oocyte not able to develop to the blastocyst stage (Bl-), able to develop to the blastocyst stage but failing to establish a pregnancy (P-), or able to develop to the blastocyst stage and to establish a clinical pregnancy (P+).RESULTS:
The cumulus cell trancriptome was largely independent of the developmental potential as, using a false dscovery rate-adjusted P-value of <0.05, only 10, 11 and 5 genes were differentially expressed for the comparisons P+ versus P-, P+ versus Bl-, and P- versus Bl-, respectively, out of a total of 17,469 genes expressed. Between the differentially expressed genes, those showing little overlap between samples from different groups were CHAC1, up-regulated in the P- and P+ groups compared with the Bl- group, and CENPE, CD93, PECAM1 and HSPA1B, which showed the opposite expression pattern. Focusing on the pregnancy potential, only EPN3 was consistently downregulated in the P+ compared with the P- and Bl- groups.CONCLUSIONS:
The cumulus cell transcriptome is largely unrelated to the establishment of clinical pregnancy following embryo transfer, although the expression level of a subset of genes in cumulus cells may indicate the ability to develop to the blastocyst stage.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cumulus Cells
/
Transcriptome
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
Reprod Biomed Online
Journal subject:
MEDICINA REPRODUTIVA
Year:
2023
Document type:
Article
Affiliation country:
Spain