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PRMT5/FGFR3/AKT Signaling Axis Facilitates Lung Cancer Cell Metastasis.
Zheng, Yonghua; Lu, Jingjing; Hu, Xiaoyan; Hu, Xiaobiao; Gao, Xiwen; Zhou, Jie.
Affiliation
  • Zheng Y; Department of Pulmonary Medicine, Shanghai Jinshan Tinglin Hospital, Shanghai, P.R. China.
  • Lu J; Department of Pulmonary and Critical Care Medicine, 66324Shanghai East Hospital, Tongji University School of Medicine, Shanghai, P.R. China.
  • Hu X; Department of Pulmonary Medicine, Shanghai Jinshan Tinglin Hospital, Shanghai, P.R. China.
  • Hu X; Department of Pulmonary Medicine, Shanghai Jinshan Tinglin Hospital, Shanghai, P.R. China.
  • Gao X; Department of Pulmonary and Critical Care Medicine, Minhang Hospital, Fudan University, Shanghai, P.R. China.
  • Zhou J; Department of Pulmonary and Critical Care Medicine, Minhang Hospital, Fudan University, Shanghai, P.R. China.
Technol Cancer Res Treat ; 22: 15330338231161139, 2023.
Article in En | MEDLINE | ID: mdl-36927233
Objectives: This study aims to investigate the function of the protein arginine methyltransferase 5 (PRMT5) and fibroblast growth factor receptor 3 (FGFR3)/Akt signaling axis in the epithelial-mesenchymal transition (EMT) of human lung cancer. Methods: The mRNA and protein expression levels of PRMT5, FGFR3, p-Akt, and EMT markers are determined by quantitative real-time PCR and Western blotting, respectively; the expression and localization of PRMT5, p-Akt, and proliferating cell nuclear antigen are detected by immunofluorescence; the human lung cancer cell proliferation is measured by MTS assay. Results: PRMT5 and FGFR3 are highly expressed in human lung cancer tissues and are closely related to lymphatic metastasis. Moreover, down-regulation of PRMT5 by lentivirus-mediated shRNAs or inhibition of PRMT5 by specific inhibitors attenuates FGFR3 expression, Akt phosphorylation, and lung cancer cell proliferation. Further studies show that silencing PRMT5 impairs EMT-related markers, including vimentin, collagen I, and ß-catenin. Conversely, ectopic expression of PRMT5 increases FGFR3 expression, Akt phosphorylation, and EMT-related markers, suggesting that PRMT5 regulates metastasis probably through the FGFR3/Akt signaling axis. Conclusion: PRMT5/FGFR3/Akt signaling axis controls human lung cancer progression and metastasis and also implies that PRMT5 may serve as a prognostic biomarker and therapeutic candidate for treating lung cancer.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proto-Oncogene Proteins c-akt / Lung Neoplasms Type of study: Prognostic_studies Limits: Humans Language: En Journal: Technol Cancer Res Treat Journal subject: NEOPLASIAS / TERAPEUTICA Year: 2023 Document type: Article Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proto-Oncogene Proteins c-akt / Lung Neoplasms Type of study: Prognostic_studies Limits: Humans Language: En Journal: Technol Cancer Res Treat Journal subject: NEOPLASIAS / TERAPEUTICA Year: 2023 Document type: Article Country of publication: United States