A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample.
Microbes Environ
; 38(1)2023.
Article
in En
| MEDLINE
| ID: mdl-36928278
ABSTRACT
We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h, our method simultaneously extracted high-quality DNA (peak size >10-15| |kb) and RNA (RNA integrity number >6) from lake bacterioplankton filtered samples. The method is also applicable to low-biomass samples (expected DNA or RNA yield <50| |ng) and eukaryotic microbial samples, providing an easy option for more versatile eco-genomic applications.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
RNA
Language:
En
Journal:
Microbes Environ
Year:
2023
Document type:
Article