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Sublethal Hyperthermia Transiently Disrupts Cortisol Steroidogenesis in Adrenocortical Cells.
Mullen, Nathan; Donlon, Padraig T; Sebek, Jan; Duffy, Katen; Cappiello, Grazia; Feely, Sarah; Warde, Kate M; Harhen, Brendan; Finn, David P; O'Shea, Paula M; Prakash, Punit; O'Halloran, Martin; Dennedy, Michael C.
Affiliation
  • Mullen N; Discipline of Pharmacology and Therapeutics, School of Medicine, University of Galway, Galway, H91 V4AY, Ireland.
  • Donlon PT; Discipline of Pharmacology and Therapeutics, School of Medicine, University of Galway, Galway, H91 V4AY, Ireland.
  • Sebek J; Mike Wiegers Department of Electrical and Computer Engineering, Kansas State University, Manhattan, KS 66506, USA.
  • Duffy K; Discipline of Pharmacology and Therapeutics, School of Medicine, University of Galway, Galway, H91 V4AY, Ireland.
  • Cappiello G; Translational Medical Device Laboratory, University of Galway, Galway, H91 V4AY, Ireland.
  • Feely S; Discipline of Pharmacology and Therapeutics, School of Medicine, University of Galway, Galway, H91 V4AY, Ireland.
  • Warde KM; Discipline of Pharmacology and Therapeutics, School of Medicine, University of Galway, Galway, H91 V4AY, Ireland.
  • Harhen B; Huntsman Cancer Institute, Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
  • Finn DP; Discipline of Pharmacology and Therapeutics, School of Medicine, University of Galway, Galway, H91 V4AY, Ireland.
  • O'Shea PM; Discipline of Pharmacology and Therapeutics, School of Medicine, University of Galway, Galway, H91 V4AY, Ireland.
  • Prakash P; Department of Clinical Biochemistry, University Hospital Galway, Newcastle Road, H91 YR71, Ireland.
  • O'Halloran M; Department of Electrical and Computer Engineering, Kansas State University, Manhattan, KS 66506, USA.
  • Dennedy MC; Translational Medical Device Laboratory, University of Galway, Galway, H91 V4AY, Ireland.
Endocrinology ; 164(5)2023 03 13.
Article in En | MEDLINE | ID: mdl-36932649
ABSTRACT
Primary aldosteronism is the most common cause of secondary hypertension. The first-line treatment adrenalectomy resects adrenal nodules and adjacent normal tissue, limiting suitability to those who present with unilateral disease. Use of thermal ablation represents an emerging approach as a possible minimally invasive therapy for unilateral and bilateral disease, to target and disrupt hypersecreting aldosterone-producing adenomas, while preserving adjacent normal adrenal cortex. To determine the extent of damage to adrenal cells upon exposure to hyperthermia, the steroidogenic adrenocortical cell lines H295R and HAC15 were treated with hyperthermia at temperatures between 37 and 50°C with the effects of hyperthermia on steroidogenesis evaluated following stimulation with forskolin and ANGII. Cell death, protein/mRNA expression of steroidogenic enzymes and damage markers (HSP70/90), and steroid secretion were analyzed immediately and 7 days after treatment. Following treatment with hyperthermia, 42°C and 45°C did not induce cell death and were deemed sublethal doses while ≥50°C caused excess cell death in adrenal cells. Sublethal hyperthermia (45°C) caused a significant reduction in cortisol secretion immediately following treatment while differentially affecting the expression of various steroidogenic enzymes, although recovery of steroidogenesis was evident 7 days after treatment. As such, sublethal hyperthermia, which occurs in the transitional zone during thermal ablation induces a short-lived, unsustained inhibition of cortisol steroidogenesis in adrenocortical cells in vitro.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Adrenal Cortex / Adrenocortical Adenoma / Hyperthermia, Induced Limits: Humans Language: En Journal: Endocrinology Year: 2023 Document type: Article Affiliation country: Ireland

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Adrenal Cortex / Adrenocortical Adenoma / Hyperthermia, Induced Limits: Humans Language: En Journal: Endocrinology Year: 2023 Document type: Article Affiliation country: Ireland