Ultrafine V2O5-anchored 3D N-doped carbon nanocomposite with augmented dual-enzyme mimetic activity for evaluating total antioxidant capacity.
Anal Chim Acta
; 1252: 341072, 2023 Apr 29.
Article
in En
| MEDLINE
| ID: mdl-36935159
ABSTRACT
Total antioxidant capacity (TAC) can be evaluated by detecting the content of antioxidants, such as ascorbic acid, based on the enzyme-mimetic activity of nanomaterials. Herein, we fabricated a 3D-V2O5/NC nanocomposite using a self-templating strategy, which achieved ultrafine particles (â¼2.5 nm), a porous carbon layer, large specific surface area (152.4 m2/g), N-doping and heterogeneous structure. The strong catalytic activity of 3D-V2O5/NC resulted from the integrated effect between the ultrafine structure of V2O5 nanoparticles and the 3D porous nitrogen-doped carbon framework, effectively increasing the number of active sites. This nanozyme presented a higher catalytic activity than its components or precursors in the nanocomposite (e.g., VN/NC, NC, V2O5, and VO2/g-C3N4). ROS scavenging experiments confirmed that the dual enzyme-like activity of 3D-V2O5/NC (catalase-like and oxidase-like) resulted from their co-participation of â§O2-, h+ and â§OH, among which â§O2- played a crucial role in the catalytic color reaction. By virtue of the 3D-V2O5/NC nanoenzyme activity and TMB as a chromogenic substrate, the mixed system of 3D-V2O5/NC + TMB + H2O2 provided a low detection limit (0.03 µM) and suitable recovery (93.0-109.5%) for AA. Additionally, a smartphone-based colorimetric application was developed employing "Thing Identify" software to evaluate TAC in beverages. The colorimetric sensor and smartphone-detection platform provide a better or comparable analytical performance for TAC assessment in comparison to commercial ABTS test kits. The newly developed smartphone-based colorimetric platform presents several prominent advantageous, such as low cost, simple/rapid operation, and feasibility for outdoor use.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nanocomposites
/
Antioxidants
Language:
En
Journal:
Anal Chim Acta
Year:
2023
Document type:
Article
Affiliation country:
China