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The Chlamydomonas reinhardtii chloroplast envelope protein LCIA transports bicarbonate in planta.
Förster, Britta; Rourke, Loraine M; Weerasooriya, Hiruni N; Pabuayon, Isaiah C M; Rolland, Vivien; Au, Eng Kee; Bala, Soumi; Bajsa-Hirschel, Joanna; Kaines, Sarah; Kasili, Remmy W; LaPlace, Lillian M; Machingura, Marylou C; Massey, Baxter; Rosati, Viviana C; Stuart-Williams, Hilary; Badger, Murray R; Price, G Dean; Moroney, James V.
Affiliation
  • Förster B; The Australian National University, Canberra, ACT 2600, Australia.
  • Rourke LM; The Australian National University, Canberra, ACT 2600, Australia.
  • Weerasooriya HN; Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
  • Pabuayon ICM; Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
  • Rolland V; CSIRO Agriculture and Food, Canberra, ACT 2601, Australia.
  • Au EK; The Australian National University, Canberra, ACT 2600, Australia.
  • Bala S; The Australian National University, Canberra, ACT 2600, Australia.
  • Bajsa-Hirschel J; Natural Products Utilization Research Unit, United States Department of Agriculture, University, MS 38677, USA.
  • Kaines S; The Australian National University, Canberra, ACT 2600, Australia.
  • Kasili RW; Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
  • LaPlace LM; Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
  • Machingura MC; Biology Department, Georgia Southern University, Savannah, GA 31419, USA.
  • Massey B; The Australian National University, Canberra, ACT 2600, Australia.
  • Rosati VC; Department of Biology, Centre for Novel Agricultural Products (CNAP), University of York, Wentworth Way, York YO10 5DD, UK.
  • Stuart-Williams H; The Australian National University, Canberra, ACT 2600, Australia.
  • Badger MR; The Australian National University, Canberra, ACT 2600, Australia.
  • Price GD; The Australian National University, Canberra, ACT 2600, Australia.
  • Moroney JV; Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
J Exp Bot ; 74(12): 3651-3666, 2023 06 27.
Article in En | MEDLINE | ID: mdl-36987927
ABSTRACT
LCIA (low CO2-inducible protein A) is a chloroplast envelope protein associated with the CO2-concentrating mechanism of the green alga Chlamydomonas reinhardtii. LCIA is postulated to be a HCO3- channel, but previous studies were unable to show that LCIA was actively transporting bicarbonate in planta. Therefore, LCIA activity was investigated more directly in two heterologous systems an Escherichia coli mutant (DCAKO) lacking both native carbonic anhydrases and an Arabidopsis mutant (ßca5) missing the plastid carbonic anhydrase ßCA5. Neither DCAKO nor ßca5 can grow in ambient CO2 conditions, as they lack carbonic anhydrase-catalyzed production of the necessary HCO3- concentration for lipid and nucleic acid biosynthesis. Expression of LCIA restored growth in both systems in ambient CO2 conditions, which strongly suggests that LCIA is facilitating HCO3- uptake in each system. To our knowledge, this is the first direct evidence that LCIA moves HCO3- across membranes in bacteria and plants. Furthermore, the ßca5 plant bioassay used in this study is the first system for testing HCO3- transport activity in planta, an experimental breakthrough that will be valuable for future studies aimed at improving the photosynthetic efficiency of crop plants using components from algal CO2-concentrating mechanisms.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chlamydomonas reinhardtii / Carbonic Anhydrases Language: En Journal: J Exp Bot Journal subject: BOTANICA Year: 2023 Document type: Article Affiliation country: Australia
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chlamydomonas reinhardtii / Carbonic Anhydrases Language: En Journal: J Exp Bot Journal subject: BOTANICA Year: 2023 Document type: Article Affiliation country: Australia