NCR negative group 3 innate lymphoid cell (NCR- ILC3) participates in abnormal pathology of lung in cigarette smoking-induced COPD mice.
Immun Inflamm Dis
; 11(3): e816, 2023 03.
Article
in En
| MEDLINE
| ID: mdl-36988250
ABSTRACT
BACKGROUND:
Natural cytotoxicity receptor negative innate lymphoid cell (NCR- ILC3) involves into mucosal homeostasis, inflammation regulation and tissue remodeling. The proportion of NCR- ILC3 is increased in the lung of smokers with chronic obstructive pulmonary disease (COPD). However, there's still few understandings on the role of NCR- ILC3 in COPD pathogenesis.METHODS:
COPD mice were induced by cigarette smoking. The pathology in lung was detected in histology. The frequency of NCR- ILC3 (CD3-CD45+RORγt+NkP46-) from murine lung was detected using flow cytometry. IL-17+RORγt+ double positive cells in lung were assessed by double immunofluorescence staining. The protein expressions of epithelial-to-mesenchymal transition (EMT) markers, namely E-cadherin and Vimentin, were assessed using immunohistochemistry staining and western blotting.RESULTS:
The frequency of NCR- ILC3 in lung was higher in COPD group than controls. The IL-17+RORγt+ cells in lung from COPD mice were more than controls. E-cadherin expression was decreased but Vimentin expression was increased in lung of COPD mice, when compared with controls. The frequency of NCR- ILC3 in lung tissues were positively correlated with mean linear intercept in lung, destructive index in lung and EMT, respectively.CONCLUSIONS:
NCR- ILC3 could contribute to emphysema and EMT in lung of cigarette smoking-induced COPD, which will provide further understanding on COPD pathogenesis of immune response.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pulmonary Disease, Chronic Obstructive
/
Cigarette Smoking
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Immun Inflamm Dis
Year:
2023
Document type:
Article
Affiliation country:
China
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM