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Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.).
Quach, Truyen; Nguyen, Hanh; Meyer, Olivia; Sato, Shirley J; Clemente, Tom Elmo; Guo, Ming.
Affiliation
  • Quach T; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
  • Nguyen H; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
  • Meyer O; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA.
  • Sato SJ; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
  • Clemente TE; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA.
  • Guo M; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA. mguo2@unl.edu.
Methods Mol Biol ; 2653: 273-285, 2023.
Article in En | MEDLINE | ID: mdl-36995632
Cas9-based genome editing is a powerful genetic tool for loci specifically targeted for genome modification. This chapter describes up-to-date protocols using Cas9-based genome editing technology, including vector construction with GoldenBraid assembly, Agrobacterium-mediated soybean transformation, and identification of editing in the genome.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Glycine max / Gene Editing Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2023 Document type: Article Affiliation country: United States Country of publication: United States
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Glycine max / Gene Editing Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2023 Document type: Article Affiliation country: United States Country of publication: United States