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Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer.
Heaps, Amy L; Sobolewski, Michele D; Jacobs, Jana L; Gordon, Kelley C; Haidar, Ghady; Mellors, John W; Parikh, Urvi M.
Affiliation
  • Heaps AL; University of Pittsburgh School of Medicine, Department of Infectious Diseases, USA.
  • Sobolewski MD; University of Pittsburgh School of Medicine, Department of Infectious Diseases, USA.
  • Jacobs JL; University of Pittsburgh School of Medicine, Department of Infectious Diseases, USA.
  • Gordon KC; University of Pittsburgh School of Medicine, Department of Infectious Diseases, USA.
  • Haidar G; University of Pittsburgh School of Medicine, Department of Infectious Diseases, USA.
  • Mellors JW; University of Pittsburgh School of Medicine, Department of Infectious Diseases, USA.
  • Parikh UM; University of Pittsburgh School of Medicine, Department of Infectious Diseases, USA. Electronic address: ump3@pitt.edu.
J Virol Methods ; 316: 114726, 2023 06.
Article in En | MEDLINE | ID: mdl-36996908
ABSTRACT
Accurate and rapid evaluation of SARS-CoV-2 half-maximal neutralizing antibody (nAb) titer (NT50) is an important research tool for measuring nAb responses after prophylaxis or therapeutics for COVID-19 prevention and management. Compared with ACE2-competitive enzyme immunoassays for nAb detection, pseudovirus assays remain low-throughput and labor intensive. A novel application of the Bio-Rad Bio-Plex Pro Human SARS-CoV-2 D614G S1 Variant nAb Assay was used to determine NT50 from COVID-19-vaccinated individuals and showed strong correlation to a laboratory-developed SARS-CoV-2 pseudovirus nAb assay. The Bio-Plex nAb assay could provide a rapid, high-throughput, culture-free method for NT50 determination in sera.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA Viruses / COVID-19 Type of study: Diagnostic_studies Limits: Humans Language: En Journal: J Virol Methods Year: 2023 Document type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA Viruses / COVID-19 Type of study: Diagnostic_studies Limits: Humans Language: En Journal: J Virol Methods Year: 2023 Document type: Article Affiliation country: United States