FGF4 and FGF9 have synergistic effects on odontoblast differentiation.
Med Mol Morphol
; 56(3): 159-176, 2023 Sep.
Article
in En
| MEDLINE
| ID: mdl-37012505
ABSTRACT
The purpose of this study was to investigate whether fibroblast growth factor 4 (FGF4) and FGF9 are active in dentin differentiation. Dentin matrix protein 1 (Dmp1) -2A-Cre transgenic mice, which express the Cre-recombinase in Dmp1-expressing cells, were crossed with CAG-tdTomato mice as reporter mouse. The cell proliferation and tdTomato expressions were observed. The mesenchymal cell separated from neonatal molar tooth germ were cultured with or without FGF4, FGF9, and with or without their inhibitors ferulic acid and infigratinib (BGJ398) for 21 days. Their phenotypes were evaluated by cell count, flow cytometry, and real-time PCR. Immunohistochemistry for FGFR1, 2, and 3 expression and the expression of DMP1 were performed. FGF4 treatment of mesenchymal cells obtained promoted the expression of all odontoblast markers. FGF9 failed to enhance dentin sialophosphoprotein (Dspp) expression levels. Runt-related transcription factor 2 (Runx2) was upregulated until day 14 but was downregulated on day 21. Compared to Dmp1-negative cells, Dmp1-positive cells expressed higher levels of all odontoblast markers, except for Runx2. Simultaneous treatment with FGF4 and FGF9 had a synergistic effect on odontoblast differentiation, suggesting that they may play a role in odontoblast maturation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fibroblast Growth Factor 4
/
Fibroblast Growth Factor 9
/
Core Binding Factor Alpha 1 Subunit
/
Odontoblasts
Limits:
Animals
Language:
En
Journal:
Med Mol Morphol
Journal subject:
BIOLOGIA MOLECULAR
/
PATOLOGIA
Year:
2023
Document type:
Article
Affiliation country:
Japan