Adenovirus core protein V reinforces the capsid and enhances genome release from disrupted particles.
Sci Adv
; 9(14): eade9910, 2023 04 07.
Article
in En
| MEDLINE
| ID: mdl-37027464
ABSTRACT
Out of the three core proteins in human adenovirus, protein V is believed to connect the inner capsid surface to the outer genome layer. Here, we explored mechanical properties and in vitro disassembly of particles lacking protein V (Ad5-ΔV). Ad5-ΔV particles were softer and less brittle than the wild-type ones (Ad5-wt), but they were more prone to release pentons under mechanical fatigue. In Ad5-ΔV, core components did not readily diffuse out of partially disrupted capsids, and the core appeared more condensed than in Ad5-wt. These observations suggest that instead of condensing the genome, protein V antagonizes the condensing action of the other core proteins. Protein V provides mechanical reinforcement and facilitates genome release by keeping DNA connected to capsid fragments that detach during disruption. This scenario is in line with the location of protein V in the virion and its role in Ad5 cell entry.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adenoviruses, Human
/
Capsid
Limits:
Humans
Language:
En
Journal:
Sci Adv
Year:
2023
Document type:
Article
Affiliation country:
Spain