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Canine parvovirus type 2 vaccines in Brazil: Viral load in commercial vaccine vials and phylogenetic analysis of the vaccine viruses.
Lencina, Michele Machado; Truyen, Uwe; de Oliveira Santana, Weslei; Kipper, Diéssy; Delamare, Ana Paula Longaray; Paesi, Suelen; Lunge, Vagner Ricardo; Streck, André Felipe.
Affiliation
  • Lencina MM; Institute of Biotechnology, University of Caxias do Sul, 1130 Francisco Getúlio Vargas Street, Caxias do Sul, RS, 95070-560, Brazil.
  • Truyen U; Institut für Tierhygiene und Öffentliches Veterinärwesen, Leipzig Universität, Leipzig, An den Tierkliniken 1, 04103, Germany.
  • de Oliveira Santana W; Institute of Biotechnology, University of Caxias do Sul, 1130 Francisco Getúlio Vargas Street, Caxias do Sul, RS, 95070-560, Brazil.
  • Kipper D; Institute of Biotechnology, University of Caxias do Sul, 1130 Francisco Getúlio Vargas Street, Caxias do Sul, RS, 95070-560, Brazil.
  • Delamare APL; Institute of Biotechnology, University of Caxias do Sul, 1130 Francisco Getúlio Vargas Street, Caxias do Sul, RS, 95070-560, Brazil.
  • Paesi S; Institute of Biotechnology, University of Caxias do Sul, 1130 Francisco Getúlio Vargas Street, Caxias do Sul, RS, 95070-560, Brazil.
  • Lunge VR; Institute of Biotechnology, University of Caxias do Sul, 1130 Francisco Getúlio Vargas Street, Caxias do Sul, RS, 95070-560, Brazil.
  • Streck AF; Institute of Biotechnology, University of Caxias do Sul, 1130 Francisco Getúlio Vargas Street, Caxias do Sul, RS, 95070-560, Brazil. Electronic address: afstreck@ucs.br.
Biologicals ; 82: 101676, 2023 May.
Article in En | MEDLINE | ID: mdl-37028214
ABSTRACT
Canine parvovirus type 2 (CPV-2) is the etiological agent of a highly contagious and frequently fatal disease in dogs. Live attenuated vaccines (LAV) are recommended to prevent and control this disease. Commercial vaccines are typically produced with CPV-2 strains adapted to cell culture and usually non-pathogenic. The present study aimed to determine the viral load of CPV-2 vaccines commercially available in Brazil and to characterize the vaccine virus by DNA analysis of its capsid gene. The results demonstrated that all vaccine strains presented high homology of the VP2 gene and they were all closely related to the original CPV-2 strains. However, vaccine strains presented several differences in comparison with field strains currently circulating in Brazil. Seventy-one vials contained viral loads ranging from 7.4E3 to 4.9E10 DNA copies/ml. Nine vials did not contain any detectable CPV-2 DNA. In conclusion, there are genetic and antigenic differences among CPV-2 vaccines and field strains. Additionally, some vaccines have been commercialized with low titers of CPV-2. It is important to improve the quality of the vaccines to prevent or reduce the spread of CPV-2 in Brazil.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Parvovirus, Canine / Parvoviridae Infections / Dog Diseases Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: Biologicals Journal subject: ALERGIA E IMUNOLOGIA Year: 2023 Document type: Article Affiliation country: Brazil
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Parvovirus, Canine / Parvoviridae Infections / Dog Diseases Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: Biologicals Journal subject: ALERGIA E IMUNOLOGIA Year: 2023 Document type: Article Affiliation country: Brazil