Antimicrobial photodynamic therapy: modern technology in the treatment of wound infections in patients with burns.
J Wound Care
; 32(Sup4a): xxxi-xxxviii, 2023 Apr 01.
Article
in En
| MEDLINE
| ID: mdl-37029985
ABSTRACT
BACKGROUND:
Staphylococcus aureus is one of the most frequently isolated microorganisms from burn wounds. Antimicrobial photodynamic therapy (aPDT) is a new strategy that may improve antimicrobial treatment.METHOD:
This study evaluated three meticillin-resistant Staphylococcus aureus (MRSA) and three meticillin-sensitive Staphylococcus aureus (MSSA) clinical isolates, which produced a biofilm with 0.1mg/ml Toluidine Blue O (TBO) (Sigma-Aldrich, Germany) with an energy density of 45J/cm2 and 90J/cm2, for MRSA and MSSA, respectively. The antibiofilm potential of aPDT with TBO was analysed using crystal violet assays and scanning electron microscopy.RESULTS:
TBO-aPDT significantly degraded the biofilm formed by MRSA and MSSA clinical isolates (p<0.05).CONCLUSION:
Our results indicated that aPDT is an effective approach to combat bacterial biofilms associated with burn wound infection. aPDT could provide a supplemental to the treatment of wound and tissue infection, and patients with burns may benefit from combined treatments.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Photochemotherapy
/
Staphylococcal Infections
/
Wound Infection
/
Burns
/
Methicillin-Resistant Staphylococcus aureus
/
Anti-Infective Agents
Limits:
Humans
Language:
En
Journal:
J Wound Care
Journal subject:
ENFERMAGEM
Year:
2023
Document type:
Article
Affiliation country:
Iran