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A lab-on-a-chip utilizing microwaves for bacterial spore disruption and detection.
Valijam, Shayan; Nilsson, Daniel P G; Öberg, Rasmus; Jonsmoen, Unni Lise Albertsdóttir; Porch, Adrian; Andersson, Magnus; Malyshev, Dmitry.
Affiliation
  • Valijam S; Faculty of Electrical Engineering, K. N. Toosi University of Technology, Tehran, 1631714191, Iran; Department of Physics, Umeå University, Umeå, 901 87, Sweden.
  • Nilsson DPG; Department of Physics, Umeå University, Umeå, 901 87, Sweden.
  • Öberg R; Department of Physics, Umeå University, Umeå, 901 87, Sweden.
  • Jonsmoen ULA; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, 1433, Norway.
  • Porch A; School of Engineering, Cardiff University, Cardiff, CF24 3AA, United Kingdom.
  • Andersson M; Department of Physics, Umeå University, Umeå, 901 87, Sweden; Umeå Center for Microbial Research (UCMR), Umeå, 901 87, Sweden. Electronic address: magnus.andersson@umu.se.
  • Malyshev D; Department of Physics, Umeå University, Umeå, 901 87, Sweden. Electronic address: dmitry.malyshev@umu.se.
Biosens Bioelectron ; 231: 115284, 2023 Jul 01.
Article in En | MEDLINE | ID: mdl-37031508
Bacterial spores are problematic in agriculture, the food industry, and healthcare, with the fallout costs from spore-related contamination being very high. Spores are difficult to detect since they are resistant to many of the bacterial disruption techniques used to bring out the biomarkers necessary for detection. Because of this, effective and practical spore disruption methods are desirable. In this study, we demonstrate the efficiency of a compact microfluidic lab-on-chip built around a coplanar waveguide (CPW) operating at 2.45 GHz. We show that the CPW generates an electric field hotspot of ∼10 kV/m, comparable to that of a commercial microwave oven, while using only 1.2 W of input power and thus resulting in negligible sample heating. Spores passing through the microfluidic channel are disrupted by the electric field and release calcium dipicolinic acid (CaDPA), a biomarker molecule present alongside DNA in the spore core. We show that it is possible to detect this disruption in a bulk spore suspension using fluorescence spectroscopy. We then use laser tweezers Raman spectroscopy (LTRS) to show the loss of CaDPA on an individual spore level and that the loss increases with irradiation power. Only 22% of the spores contain CaDPA after exposure to 1.2 W input power, compared to 71% of the untreated control spores. Additionally, spores exposed to microwaves appear visibly disrupted when imaged using scanning electron microscopy (SEM). Overall, this study shows the advantages of using a CPW for disrupting spores for biomarker release and detection.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Spores, Bacterial / Microbiological Techniques / Lab-On-A-Chip Devices / Microwaves Type of study: Diagnostic_studies Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2023 Document type: Article Affiliation country: Sweden Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Spores, Bacterial / Microbiological Techniques / Lab-On-A-Chip Devices / Microwaves Type of study: Diagnostic_studies Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2023 Document type: Article Affiliation country: Sweden Country of publication: United kingdom