Endocytic proteins mediating GPR15 receptor internalization provide insight into the underlying mechanisms.
FEBS Lett
; 597(11): 1528-1540, 2023 06.
Article
in En
| MEDLINE
| ID: mdl-37051832
ABSTRACT
GPR15 is a G protein-coupled receptor involved in immune disorders such as human immunodeficiency virus-induced enteropathy, multiple sclerosis, and colitis. Yet, the important endocytosis mechanism of GPR15 remained unclear. This study determined the participation of endocytic machinery proteins, including Gα proteins, G protein-coupled receptor kinases (GRKs), protein kinase C, arrestins, clathrin, caveolin, and dynamin in GPR15 internalization. The results demonstrate that GPR15 internalization is moderately dependent on GRKs and clathrin, and highly dependent on caveolin and dynamin. Moreover, a bystander arrestin recruitment assay showed that GPR15 recruits arrestin-3 to the cell membrane upon agonist stimulation, although GPR15 internalizes in an arrestin-independent manner. Overall, our study provides novel insights into ß-arrestin recruitment and receptor internalization mechanisms for the recently deorphanized GPR15.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arrestin
/
Receptors, G-Protein-Coupled
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
2023
Document type:
Article
Affiliation country:
Denmark