NanoBiT- and NanoBiT/BRET-based assays allow the analysis of binding kinetics of Wnt-3a to endogenous Frizzled 7 in a colorectal cancer model.

Grätz, Lukas; Sajkowska-Kozielewicz, Joanna J; Wesslowski, Janine; Kinsolving, Julia; Bridge, Lloyd J; Petzold, Katja; Davidson, Gary; Schulte, Gunnar; Kozielewicz, Pawel

Grätz, Lukas; Sajkowska-Kozielewicz, Joanna J; Wesslowski, Janine; Kinsolving, Julia; Bridge, Lloyd J; Petzold, Katja; Davidson, Gary; Schulte, Gunnar; Kozielewicz, Pawel.

Affiliation

Grätz L; Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
Sajkowska-Kozielewicz JJ; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Wesslowski J; Department of Organic and Physical Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
Kinsolving J; Institute of Biological and Chemical Systems-Functional Molecular Systems, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Bridge LJ; Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
Petzold K; Department of Computer Science and Creative Technologies, University of the West England, Bristol, UK.
Davidson G; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Schulte G; Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Kozielewicz P; Institute of Biological and Chemical Systems-Functional Molecular Systems, Karlsruhe Institute of Technology, Karlsruhe, Germany.

Br J Pharmacol ; 2023 Apr 13.

Article in En | MEDLINE | ID: mdl-37055379

Key words

CRISPR-Cas9; FZD7; NanoBiT/BRET binding; SW480 cells; Wnt; binding affinity shift

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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Br J Pharmacol Year: 2023 Document type: Article Affiliation country: Sweden Country of publication: United kingdom

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