Roles of Optineurin and Extracellular Vesicles in Glaucomatous Retinal Cell Loss.
Curr Med Sci
; 43(2): 367-375, 2023 Apr.
Article
in En
| MEDLINE
| ID: mdl-37059934
ABSTRACT
OBJECTIVE:
To explore the role of extracellular vesicles (EVs) in the pathogenesis of glaucoma caused by E50K mutation.METHODS:
A photoreceptor cell line, RGC-5, was transfected with empty plasmids and plasmids expressing wild-type (WT) optineurin (OPTN) or E50K OPTN to investigate the effects of OPTN glaucoma as well as to identify the role of EVs in glaucoma pathology. The RGC-5 cells were also stimulated with glutamate, and their viability was evaluated using flow cytometry or CCK-8 assay. EVs were extracted, labeled with PKH-26, and added into the medium for normal RGC-5 culture, and the status of the cells was observed thereafter.RESULTS:
WT OPTN overexpression, E50K OPTN, and glutamate stimulation induced apoptosis of RGC-5 cells. However, when glutamate stimulation was used as an add-on treatment, the degree of apoptosis in WT OPTN-overexpressing RGC-5 cells was significantly lower than that in E50K OPTN-expressing and normal RGC-5 cells. The viability of normal RGC-5 cells was reduced when co-cultured with WT OPTN-overexpressing RGC-5 or E50K OPTN-overexpressing RGC-5. EVs released by the latter two transfected lines similarly reduced normal RGC-5 survival.CONCLUSION:
Our results indicate that WT OPTN overexpression may lead to photoreceptor apoptosis. However, overexpression also confers a degree of protection against high concentrations of extracellular glutamate. Additionally, EVs released by transfected RGC-5 cells may regulate the cell state. These findings may improve our understanding of the mechanisms of cell-cell interactions in pathological conditions, providing a basis for the use of EVs as novel targets for early diagnosis and treatment of glaucoma.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glaucoma
/
Extracellular Vesicles
Type of study:
Prognostic_studies
/
Screening_studies
Limits:
Humans
Language:
En
Journal:
Curr Med Sci
Year:
2023
Document type:
Article
Affiliation country:
China