Enzyme Inhibition and Antioxidant Activities of Asparagus officinalis L. and Analysis of Its Phytochemical Content by LC/MS/MS.
Chem Biodivers
; 20(5): e202201231, 2023 May.
Article
in En
| MEDLINE
| ID: mdl-37096958
ABSTRACT
In the study, water, ethanol, methanol, dichloromethane, and acetone extracts of Asparagus officinalis L. were obtained by maceration. DPPHâ
, ABTSâ
+ , FRAP, and CUPRAC methods determined the antioxidant capacities of all extracts. Moreover, the inâ
vitro effects of extracts on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase (CA)-I, CA-II and α-Glycosidase were investigated. At a 10â
µg/ml concentration, the extract with the highest Fe3+ reduction capacity was ethanol (AE), and the extract with the highest Cu2+ reduction capacity was acetone (AA). AE for AChE (IC50 =21.19â
µg/ml) and α-Glycosidase (IC50 70.00â
µg/ml), methanol (AM) for BChE (IC50 =17.33â
µg/ml), CA-I and II (IC50 =79.65 and 36.09â
µg/ml, respectively) showed the most potent inhibition effect. The content analysis of acetone extract was performed with LC/MS-MS, the first three phytochemicals found most were p-Coumaric acid, rutin, and 4-hydroxybenzoic acid (284.29±3.97, 135.39±8.19, and 102.06±5.51â
µg analyte/g extract, respectively).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Asparagus Plant
/
Antioxidants
Language:
En
Journal:
Chem Biodivers
Journal subject:
BIOQUIMICA
/
QUIMICA
Year:
2023
Document type:
Article
Publication country:
CH
/
SUIZA
/
SUÍÇA
/
SWITZERLAND