An origami-based colorimetric sensor for detection of hydrogen peroxide and glucose using sericin capped silver nanoparticles.
Sci Rep
; 13(1): 7064, 2023 05 01.
Article
in En
| MEDLINE
| ID: mdl-37127668
ABSTRACT
The hydrogen peroxide (H2O2) measurement is considered highly important in industrial wastewater quality assessment, environmental protection, and disease detection. Here, a simple high-performance paper-based sensor is proposed for rapid and in situ detection of H2O2. To this end, 3,3',5,5'-tetramethylbenzidine is embedded in the sensor to act as a color indicator, whose reaction with hydrogen peroxide is catalyzed by a silver nanozyme modified by sericin. The result of the reaction clarified by the appearance of blue color in the sensor detection zone is received by a portable scanner, while also calculating its intensity by image analysis software. This method is sensitive to hydrogen peroxide in the concentration range of 0.5â240 mg/dL, providing a detection limit of 0.15 mg/dL. The ability of the sensor to determine glucose is also evaluated by adding a layer containing glucose oxidase enzyme to the sensor structure. A desirable response is obtained in the range of 1.0â160 mg/dL, together with a detection limit of 0.37 mg/dL. Accordingly, the proposed sensor shows satisfactory results compared to clinical methods for monitoring the amount of glucose in biological samples such as serum and saliva.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sericins
/
Metal Nanoparticles
Type of study:
Diagnostic_studies
Language:
En
Journal:
Sci Rep
Year:
2023
Document type:
Article
Affiliation country:
Iran