Comparison of 4 agar gel immunodiffusion kits for serologic detection of equine infectious anemia virus antibodies.
J Vet Diagn Invest
; 35(4): 430-432, 2023 Jul.
Article
in En
| MEDLINE
| ID: mdl-37129383
ABSTRACT
Using 85 sera collected from horses that had been experimentally infected with equine infectious anemia virus (EIAV) and 200 field sera collected from racehorses in Japan, we compared 4 agar gel immunodiffusion (AGID) kits for serologic detection of EIAV antibodies from Idexx, VMRD, IDvet, and the National Engineering Research Center of Veterinary Biologics, China (NECVB). The positive control lines were sufficiently clear in all kits for evaluation to be made, with slight differences in sharpness NECVB was the sharpest, followed by VMRD, IDvet, and Idexx. The test results for all 285 samples agreed among the 4 kits, with 62 positives and 223 negatives. The sensitivities and specificities of VMRD, IDvet, and NECVB compared with the Idexx kit were 100%, and the kappa coefficient values between the kits were 1.0 for all combinations. We concluded that the testing capacity of these 4 kits was virtually identical.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Equine Infectious Anemia
/
Infectious Anemia Virus, Equine
/
Horse Diseases
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Vet Diagn Invest
Journal subject:
MEDICINA VETERINARIA
Year:
2023
Document type:
Article
Affiliation country:
Japan