Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system.
J Reprod Dev
; 69(4): 214-217, 2023 Aug 11.
Article
in En
| MEDLINE
| ID: mdl-37197977
ABSTRACT
In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF). The proportion of oocytes that progressed to the germinal vesicle breakdown stage was similar between groups with and without pre-IVM. Although metaphase II oocytes and cleavage rates after IVF were similar regardless of pre-IVM culture, the blastocyst rate was significantly higher in the group with pre-IVM (22.5%) than without pre-IVM (11.0%, P < 0.05). In conclusion, pre-IVM culture improved the developmental competence of bovine oocytes derived from an 8-day IVG system.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oocytes
/
Fertilization in Vitro
Limits:
Animals
Language:
En
Journal:
J Reprod Dev
Journal subject:
MEDICINA REPRODUTIVA
Year:
2023
Document type:
Article
Affiliation country:
Japan
Publication country:
JAPAN
/
JAPON
/
JAPÃO
/
JP