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Calcium-triggered DNA-mediated membrane fusion in synthetic cells.
Hsu, Yen-Yu; Chen, Samuel J; Bernal-Chanchavac, Julio; Sharma, Bineet; Moghimianavval, Hossein; Stephanopoulos, Nicholas; Liu, Allen P.
Affiliation
  • Hsu YY; Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
  • Chen SJ; Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
  • Bernal-Chanchavac J; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Sharma B; Department of Chemistry and Chemical Biology, Rutgers University, New Brunswick, New Jersey, USA.
  • Moghimianavval H; Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
  • Stephanopoulos N; School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
  • Liu AP; Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
bioRxiv ; 2023 Jun 02.
Article in En | MEDLINE | ID: mdl-37205334
ABSTRACT
In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is regulated using surface-bound PEG chains that are cleavable by the calcium-activated protease calpain-1.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: BioRxiv Year: 2023 Document type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: BioRxiv Year: 2023 Document type: Article Affiliation country: United States