Mass spectrometry-based phosphoproteomics in clinical applications.
Trends Analyt Chem
; 1632023 Jun.
Article
in En
| MEDLINE
| ID: mdl-37215489
ABSTRACT
Protein phosphorylation is an essential post-translational modification that regulates many aspects of cellular physiology, and dysregulation of pivotal phosphorylation events is often responsible for disease onset and progression. Clinical analysis on disease-relevant phosphoproteins, while quite challenging, provides unique information for precision medicine and targeted therapy. Among various approaches, mass spectrometry (MS)-centered characterization features discovery-driven, high-throughput and in-depth identification of phosphorylation events. This review highlights advances in sample preparation and instrument in MS-based phosphoproteomics and recent clinical applications. We emphasize the preeminent data-independent acquisition method in MS as one of the most promising future directions and biofluid-derived extracellular vesicles as an intriguing source of the phosphoproteome for liquid biopsy.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Trends Analyt Chem
Year:
2023
Document type:
Article
Affiliation country:
United States