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Modularity and diversity of target selectors in Tn7 transposons.
Faure, Guilhem; Saito, Makoto; Benler, Sean; Peng, Iris; Wolf, Yuri I; Strecker, Jonathan; Altae-Tran, Han; Neumann, Edwin; Li, David; Makarova, Kira S; Macrae, Rhiannon K; Koonin, Eugene V; Zhang, Feng.
Affiliation
  • Faure G; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Tech
  • Saito M; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Tech
  • Benler S; National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.
  • Peng I; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Tech
  • Wolf YI; National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.
  • Strecker J; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Tech
  • Altae-Tran H; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Tech
  • Neumann E; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Tech
  • Li D; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Tech
  • Makarova KS; National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.
  • Macrae RK; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Tech
  • Koonin EV; National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA. Electronic address: koonin@ncbi.nlm.nih.gov.
  • Zhang F; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Tech
Mol Cell ; 83(12): 2122-2136.e10, 2023 Jun 15.
Article in En | MEDLINE | ID: mdl-37267947
ABSTRACT
To spread, transposons must integrate into target sites without disruption of essential genes while avoiding host defense systems. Tn7-like transposons employ multiple mechanisms for target-site selection, including protein-guided targeting and, in CRISPR-associated transposons (CASTs), RNA-guided targeting. Combining phylogenomic and structural analyses, we conducted a broad survey of target selectors, revealing diverse mechanisms used by Tn7 to recognize target sites, including previously uncharacterized target-selector proteins found in newly discovered transposable elements (TEs). We experimentally characterized a CAST I-D system and a Tn6022-like transposon that uses TnsF, which contains an inactivated tyrosine recombinase domain, to target the comM gene. Additionally, we identified a non-Tn7 transposon, Tsy, encoding a homolog of TnsF with an active tyrosine recombinase domain, which we show also inserts into comM. Our findings show that Tn7 transposons employ modular architecture and co-opt target selectors from various sources to optimize target selection and drive transposon spread.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Transposable Elements / Clustered Regularly Interspaced Short Palindromic Repeats Type of study: Prognostic_studies / Qualitative_research Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2023 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Transposable Elements / Clustered Regularly Interspaced Short Palindromic Repeats Type of study: Prognostic_studies / Qualitative_research Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2023 Document type: Article