Generation of liver mesenchyme and ductal cell organoid co-culture using cell self-aggregation and droplet microfluidics.
STAR Protoc
; 4(2): 102333, 2023 Jun 02.
Article
in En
| MEDLINE
| ID: mdl-37270780
ABSTRACT
Within the peri-portal region of the adult liver, portal fibroblasts exist in close proximity to epithelial ductal/cholangiocyte cells. However, the cellular interactions between them are poorly understood. Here, we provide two co-culture techniques to incorporate liver portal mesenchyme into ductal cell organoids, which recapitulate aspects of their cellular interactions in vitro. We integrate several techniques from mesenchyme isolation and expansion to co-culture by microfluidic cell co-encapsulation or 2D-Matrigel layer. The protocol is easily adaptable to other cells from other organs. For complete information on the generation and use of this protocol, please refer to Cordero-Espinoza et al.1.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
STAR Protoc
Year:
2023
Document type:
Article
Affiliation country:
Germany