Expression of Neural Antigens in Normal Xenopus Embryos and Induced Explants: (Xenopus laevis/neural antigens/monoclonal antibodies/neural induction/neural differentiation).
Dev Growth Differ
; 31(6): 563-571, 1989 Dec.
Article
in En
| MEDLINE
| ID: mdl-37280931
ABSTRACT
Monoclonal antibodies were raised against neural tissues of Xenopus larvae. Three monoclonal antibodies, named NEU-1, NEU-3, and NEU-4, were specific for neural tissue and first bound to neural cells at stage 25 after neural tube formation (NEU-1 and NEU-3) or at stage 31 (NEU-4). These antibodies bound to differentiating neural cells, but not to germinal neuroepithelial cells. NEU-1 and NEU-3 recognized antigens in cell bodies as well as neural fibers of neural cells, and these antigens were distributed throughout the central nervous system. NEU-4 bound to antigens in granular materials in neural cells, and these antigens were present in head and trunk regions but not in the tail region. These three antibodies were used as neural markers in two types of induction experiments, in which 1) the animal pole region and the dorsal blastopore lip from stage-10 gastrulae were combined, or 2) the animal pole region and the vegetal pole region from stage-8 blastulae were combined. In both experiments, most conjugated explants expressed the NEU-1, NEU-3, and NEU-4 antigens, although the expression of NEU-4 antigen was delayed compared with those of the NEU-1 and NEU-3 antigens. These results show that these antibodies are useful as markers in neural induction experiments.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Dev Growth Differ
Year:
1989
Document type:
Article
Affiliation country:
Japan