Proton magnetic resonance studies of angiotensin II conformation: cis-trans isomerism in sarcosine7-containing analogs.
Arch Biochem Biophys
; 248(1): 419-23, 1986 Jul.
Article
in En
| MEDLINE
| ID: mdl-3729426
ABSTRACT
1H-NMR spectra for the angiotensin agonist sarcosine-(Sar)Arg-Val-Tyr-Ile-His-Sar-Phe [( Sar1,Sar7]Ang II) and the antagonist Sar-Arg-Val-Tyr-Ile-His-Sar-Ile in dimethylsulfoxide-d6 were examined at 400 MHz. Splitting of the resonances for Tyr, His, and Sar protons revealed that the His6-Sar7 peptide bond existed in both cis and trans forms, with one isomer predominating in the ratio 51 in both peptides. Comparison of the chemical shifts for the His6 and Phe8 ring protons in these peptides suggested a His/Phe stacking interaction in [Sar1,Sar7]Ang II which is important for agonist activity.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Angiotensin II
/
1-Sarcosine-8-Isoleucine Angiotensin II
Language:
En
Journal:
Arch Biochem Biophys
Year:
1986
Document type:
Article