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Simultaneous quantitation of ketamine, norketamine and dehydronorketamine in human milk using a novel ultra high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) assay.
Shahane, Aditi; Zhao, Wenchen; Pakalapati, Naren; Alshogran, Osama Y; Caritis, Steve N; Lim, Grace; Shaik, Imam H; Venkataramanan, Raman.
Affiliation
  • Shahane A; Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA.
  • Zhao W; Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA.
  • Pakalapati N; Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA.
  • Alshogran OY; Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA; Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
  • Caritis SN; University of Pittsburgh Department of Obstetrics & Gynecology, UPMC Magee-Womens Hospital, Pittsburgh, PA, USA.
  • Lim G; University of Pittsburgh Department of Obstetrics & Gynecology, UPMC Magee-Womens Hospital, Pittsburgh, PA, USA; University of Pittsburgh Department of Anesthesiology & Perioperative Medicine, Pittsburgh, PA, USA; Center for Innovation in Pain Care, University of Pittsburgh Medical Center, P
  • Shaik IH; Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA; Department of Pharmacy and Therapeutics, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA. Electronic address: ihs4@pitt.edu.
  • Venkataramanan R; Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA; Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: rv@pitt.edu.
J Pharm Biomed Anal ; 234: 115502, 2023 Sep 20.
Article in En | MEDLINE | ID: mdl-37311376
There is a paucity of data on the transfer of ketamine from maternal blood into human milk. Quantification of ketamine in human milk provides information about the potential exposure of the infant to ketamine and its metabolites from the mother during lactation. A highly specific, reproducible, and sensitive UPLC-MS/MS based analytical method was developed and validated for the quantitation of ketamine and its metabolites (norketamine and dehydronorketamine) in human milk. Samples were subjected to a simple protein precipitation and ketamine-d4 and norketamine-d4 were used as internal standards. Separation of the analytes was achieved using an Acquity UPLC equipped with BEH RP18 1.7 µm, 2.1 × 100 mm column. Mass spectrometric analysis of the analyte ions was carried out using electrospray with positive ionization and multiple reaction monitoring mode. The assay was linear over a concentration range of 1-100 ng/mL for ketamine and norketamine, and 0.1-10 ng/mL for dehydronorketamine. Acceptable intra-day and inter-day accuracy and precision were observed for all the analytes. High recovery of the analytes and minimal matrix effect were observed. Stability of analytes was confirmed at the tested conditions. This assay was successfully used to measure analytes in human milk samples collected from lactating women enrolled in a clinical research study. This is the first validated method that simultaneously quantified ketamine and its metabolites in human milk.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ketamine Type of study: Guideline Limits: Female / Humans Language: En Journal: J Pharm Biomed Anal Year: 2023 Document type: Article Affiliation country: United States Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ketamine Type of study: Guideline Limits: Female / Humans Language: En Journal: J Pharm Biomed Anal Year: 2023 Document type: Article Affiliation country: United States Country of publication: United kingdom