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In vitro antibacterial efficacy of autologous conditioned plasma and amniotic membrane eye drops.
Yates, Kelsey M; Stilwell, Natalie K; Fontenot, Robin L; Betbeze, Caroline M.
Affiliation
  • Yates KM; Department of Clinical Sciences, Mississippi State University, Mississippi State, Mississippi, USA.
  • Stilwell NK; Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, Mississippi, USA.
  • Fontenot RL; Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, Mississippi, USA.
  • Betbeze CM; Department of Clinical Sciences, Mississippi State University, Mississippi State, Mississippi, USA.
Vet Ophthalmol ; 26(6): 548-554, 2023 Nov.
Article in En | MEDLINE | ID: mdl-37317517
ABSTRACT

OBJECTIVE:

To determine the in vitro antibacterial efficacy of equine and canine autologous conditioned plasma (ACP) and amniotic membrane extract eye drops (AMEED) against aerobic bacteria common to the corneal surface. PROCEDURES Canine (n = 4) and equine (n = 4) anticoagulated whole blood samples were sterilely collected, pooled for each species, and processed using the Arthrex ACP® Double-Syringe System. Platelet counts were performed on ACP and pooled blood. AMEED were obtained from a commercial source. An electronic medical records search (2013-2022) identified aerobic bacteria cultured from canine and equine corneal ulcers at Mississippi State University College of Veterinary Medicine (MSU-CVM). Ten commonly isolated bacteria for each species were collected from cultures submitted to the MSU-CVM Microbiology Diagnostic Service and frozen at -80°C. The Kirby-Bauer disk diffusion method was used to determine the sensitivities of these isolates to ACP and AMEED. Bacterial isolates were plated onto Mueller-Hinton +5% sheep blood agar and blank sterile discs saturated with 20 µL of ACP or AMEED were tested in duplicate. Imipenem discs served as positive controls and blank discs as negative controls. Zones of inhibition were measured at 18 h.

RESULTS:

ACP platelet counts were 1.06 and 1.65 times higher than blood for equine and canine samples, respectively. Growth of a multi-drug resistant Enterococcus faecalis was partially inhibited by canine and equine ACP. AMEED did not inhibit growth of any examined bacteria.

CONCLUSIONS:

Canine and equine ACP partially inhibited E. faecalis growth in vitro. Further studies using varying concentrations of ACP against bacterial isolates from corneal ulcers are warranted.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sheep Diseases / Dog Diseases / Horse Diseases Limits: Animals / Humans Language: En Journal: Vet Ophthalmol Journal subject: MEDICINA VETERINARIA / OFTALMOLOGIA Year: 2023 Document type: Article Affiliation country: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sheep Diseases / Dog Diseases / Horse Diseases Limits: Animals / Humans Language: En Journal: Vet Ophthalmol Journal subject: MEDICINA VETERINARIA / OFTALMOLOGIA Year: 2023 Document type: Article Affiliation country: United States