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Adenovirus vector produced Zika virus-like particles induce a long-lived neutralising antibody response in mice.
Carrera, Julio; Aktepe, Turgut E; Earnest, Linda; Christiansen, Dale; Wheatley, Adam K; Tan, Hyon-Xhi; Chung, Amy W; Collett, Simon; McPherson, Kirsty; Torresi, Joseph; Mackenzie, Jason M; Simmons, Cameron P.
Affiliation
  • Carrera J; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty for Infection and Immunity, Parkville, Melbourne, VIC 3010, Australia; Institute of Vector-Borne Diseases, Monash University, Clayton, VIC 3800, Australia.
  • Aktepe TE; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty for Infection and Immunity, Parkville, Melbourne, VIC 3010, Australia.
  • Earnest L; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty for Infection and Immunity, Parkville, Melbourne, VIC 3010, Australia.
  • Christiansen D; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty for Infection and Immunity, Parkville, Melbourne, VIC 3010, Australia.
  • Wheatley AK; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty for Infection and Immunity, Parkville, Melbourne, VIC 3010, Australia.
  • Tan HX; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty for Infection and Immunity, Parkville, Melbourne, VIC 3010, Australia.
  • Chung AW; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty for Infection and Immunity, Parkville, Melbourne, VIC 3010, Australia.
  • Collett S; School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3000, Australia.
  • McPherson K; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty for Infection and Immunity, Parkville, Melbourne, VIC 3010, Australia.
  • Torresi J; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty for Infection and Immunity, Parkville, Melbourne, VIC 3010, Australia.
  • Mackenzie JM; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty for Infection and Immunity, Parkville, Melbourne, VIC 3010, Australia. Electronic address: jason.mackenzie@unimelb.edu.au.
  • Simmons CP; Institute of Vector-Borne Diseases, Monash University, Clayton, VIC 3800, Australia. Electronic address: cameron.simmons@worldmosquito.org.
Vaccine ; 41(33): 4888-4898, 2023 07 25.
Article in En | MEDLINE | ID: mdl-37391311
Countermeasures against Zika virus (ZIKV) epidemics are urgently needed. In this study we generated a ZIKV virus-like particle (VLP) based vaccine candidate and assessed the immunogenicity of these particles in mice. The ZIKV-VLPs were morphologically similar to ZIKV by electron microscopy and were recognized by anti-Flavivirus neutralising antibodies. We observed that a single dose of unadjuvanted ZIKV-VLPs, or inactivated ZIKV, generated an immune response that lasted over 6 months, but did not neutralize ZIKV infection of cells in vitro. However, when we co-administered the ZIKV VLPs with either Aluminium hydroxide (Alhydrogel®; Alum), AddaVax or Pam2Cys we observed that Alum was the most effective in a single dose regime, since it not only produced antibodies that neutralized the virus, but also generated a greater number of antigen-specific memory B cells. We additionally observed that the generation of the neutralising antibodies persisted for up to 6 months. Our results suggest that a single dose ZIKV VLPs could be a suitable single dose vaccine candidate for use in outbreak settings.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Vaccines / Zika Virus / Zika Virus Infection Limits: Animals Language: En Journal: Vaccine Year: 2023 Document type: Article Affiliation country: Australia Country of publication: Netherlands

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Vaccines / Zika Virus / Zika Virus Infection Limits: Animals Language: En Journal: Vaccine Year: 2023 Document type: Article Affiliation country: Australia Country of publication: Netherlands