Glutathione displacement assay based on a fluorescent Au(I) complex.
J Mater Chem B
; 11(31): 7344-7352, 2023 08 09.
Article
in En
| MEDLINE
| ID: mdl-37435627
ABSTRACT
Glutathione (GSH) is an essential molecule that plays a pivotal role in maintaining intracellular redox homeostasis, as well as other physiological processes. However, the chemical mechanisms underlying the GSH-induced processes remain insufficiently understood due to the lack of appropriate detection tools. Fluorescence GSH imaging can serve as a useful principle for the rapid, convenient, and non-destructive detection of GSH in living organisms. In this study, we developed a fluorescent GSH probe based on a linear, homoleptic Au(I) complex with two 1,3-diphenylbenzimidazolium carbene ligands. The Au(I) complex produced a fluorescence turn-on response to GSH. Fluorescence GSH signaling was characterized with a short response time of a few seconds. The rapid response was attributed to the displacement of the carbene ligand with GSH, which involved a labile inner-sphere coordination interaction. Finally, we demonstrated the biological utility of our GSH probe by unambiguously discriminating between different GSH levels in normal and senescent preadipocytes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glutathione
Language:
En
Journal:
J Mater Chem B
Year:
2023
Document type:
Article