Highly photoluminescent tryptophan-coated copper nanoclusters based turn-off fluorescent probe for determination of tetracyclines.

ABSTRACT

Employing cheap Cu nanoclusters to design a novel fluorescent probe have promising opportunities in the field of optical sensors. Here, we fabricated a highly photoluminescent D-tryptophan (D-Trp)-coated Cu nanoclusters (Trp-Cu NCs) by rapid microwave-assisted method to achieve precise quantification of tetracyclines (TC). Due to protecting groups of Trp, the synthesized Trp-Cu NCs have remarkable fluorescence stability with a quantum yield reached 12.5%. A distinct fluorescence quenching with the incremental addition of TC via the internal filtration effect (IFE). Based on turn-off fluorescence within 1 min, a detection method for detecting TC was constructed with a linear range of 0.3-120 µM and a limit of detection (LOD) of 0.12 µM. Besides, the proposed fluorescent probe has been employed for the determination of practical samples such as water samples, milk and honey, and exhibited satisfactory recoveries of 96.1%-108.2%, with relative standard deviations (RSD) lower than 5.0%. This is a sensitive, rapid and easily recognizable Trp-Cu NCs based sensing platform for the determination of TC, which could offer a powerful tool for ensuring food safety.