Your browser doesn't support javascript.
loading
Glucose sandwich assay based on surface-enhanced Raman spectroscopy.
Zhang, Tingting; Lu, Rui; Wang, Gongying; Sun, Xiuyun; Li, Jiansheng; Mizaikoff, Boris.
Affiliation
  • Zhang T; Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China. rlu@njust.edu.cn.
  • Lu R; Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China. rlu@njust.edu.cn.
  • Wang G; Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China. rlu@njust.edu.cn.
  • Sun X; Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China. rlu@njust.edu.cn.
  • Li J; Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China. rlu@njust.edu.cn.
  • Mizaikoff B; Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert Einstein Allee 11, 89081 Ulm, Germany.
Analyst ; 148(18): 4310-4317, 2023 Sep 11.
Article in En | MEDLINE | ID: mdl-37470091
ABSTRACT
A facile and sensitive glucose sandwich assay using surface-enhanced Raman scattering (SERS) has been developed. Glucose was captured by 3-aminopheyonyl boronic acid (APBA) modified Ag nanoparticles decorated onto a polyamide surface. Then, Ag nanoparticles modified with 3-amino-6-ethynylpicolinonitrile (AEPO) and APBA were used as SERS tags. APBA forms specific cis-diol compounds with glucose molecules avoiding interference by other saccharides and biomolecules in urine enabling its selective detection. As the actual Raman reporter, AEPO exhibited a distinctive SERS peak in the Raman silent region, thus increasing the sensitivity of the glucose detection to 10-11 M. Additionally, the developed SERS assay was reusable, and its applicability in artificial urine samples demonstrated future clinical utility confirming the potential of this innovative technology as a diagnostic tool for glucose sensing.
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Spectrum Analysis, Raman / Glucose Language: En Journal: Analyst Year: 2023 Document type: Article
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Spectrum Analysis, Raman / Glucose Language: En Journal: Analyst Year: 2023 Document type: Article