Facile Method for High-throughput Identification of Stabilizing Mutations.

Christensen, Signe; Wernersson, Camille; André, Ingemar

Christensen, Signe; Wernersson, Camille; André, Ingemar.

Affiliation

Christensen S; Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden.
Wernersson C; Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden.
André I; Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden. Electronic address: ingemar.andre@biochemistry.lu.se.

J Mol Biol ; 435(18): 168209, 2023 09 15.

Article in En | MEDLINE | ID: mdl-37479080

Subject(s)

Amino Acid Sequence; Mutation, Missense; Protein Folding; Protein Stability; Sequence Analysis, Protein; Adenylate Kinase/chemistry; Adenylate Kinase/genetics; Amino Acid Sequence/genetics; High-Throughput Screening Assays/methods; Sequence Analysis, Protein/methods; Temperature

Key words

deep mutational scanning; folding reporter; high-throughput screening; multiplex assay; thermostability

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Amino Acid Sequence / Protein Folding / Mutation, Missense / Sequence Analysis, Protein / Protein Stability Type of study: Diagnostic_studies Language: En Journal: J Mol Biol Year: 2023 Document type: Article Affiliation country: Sweden

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