Whole-body bioluminescence imaging of T-cell response in PDAC models.
Front Immunol
; 14: 1207533, 2023.
Article
in En
| MEDLINE
| ID: mdl-37497236
ABSTRACT
Introduction:
The location of T-cells during tumor progression and treatment provides crucial information in predicting the response in vivo.Methods:
Here, we investigated, using our bioluminescent, dual color, T-cell reporter mouse, termed TbiLuc, T-cell location and function during murine PDAC tumor growth and checkpoint blockade treatment with anti-PD-1 and anti-CTLA-4. Using this model, we could visualize T-cell location and function in the tumor and the surrounding tumor microenvironment longitudinally. We used murine PDAC clones that formed in vivo tumors with either high T-cell infiltration (immunologically 'hot') or low T-cell infiltration (immunologically 'cold').Results:
Differences in total T-cell bioluminescence could be seen between the 'hot' and 'cold' tumors in the TbiLuc mice. During checkpoint blockade treatment we could see in the tumor-draining lymph nodes an increase in bioluminescence on day 7 after treatment.Conclusions:
In the current work, we showed that the TbiLuc mice can be used to monitor T-cell location and function during tumor growth and treatment.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neoplasms
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Front Immunol
Year:
2023
Document type:
Article
Affiliation country:
Netherlands