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Plant Regulation Functions of Novel Phthalimide Compounds Based on AtPYL2.
Peng, Mingyao; Zhang, Shanqi; Zhao, Kunhong; Zheng, Yuguo; Li, Xiangyang.
Affiliation
  • Peng M; National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, Guizhou 550025, People's Republic of China.
  • Zhang S; College of Tea Science, Guizhou University, Huaxi District, Guiyang, Guizhou 550025, People's Republic of China.
  • Zhao K; National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, Guizhou 550025, People's Republic of China.
  • Zheng Y; National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, Guizhou 550025, People's Republic of China.
  • Li X; Key Laboratory of Chemical Synthesis and Environmental Pollution Control Remediation Technology, Minzu Normal University of Xingyi, Xingyi, Guizhou 562400, People's Republic of China.
J Agric Food Chem ; 71(32): 12325-12332, 2023 Aug 16.
Article in En | MEDLINE | ID: mdl-37534830
Novel agents contain the structure of phthalimide, which has antibacterial, insecticidal, and herbicidal activities. Recently, studies reported that these compounds can bind to plant hormone receptors and play important regulatory roles. In this study, the functions of agents were studied with in vitro and in vivo assays. The abscisic acid (ABA) receptor pyrabactin resistant-like 2 (PYL2) protein in Arabidopsis thaliana was expressed, purified, and crystallized; the analysis results of the crystal structure showed three AtPYL2 subunits in each asymmetric unit. The affinity of compounds Z1-Z11 to the AtPYL2 protein was tested by microscale thermophoresis (MST) and then verified by isothermal titration calorimetry (ITC). Furthermore, the binding pockets were found using molecular docking to verify the target relationships. Relevant in vivo assays for seed germination and a root growth assay were conducted, with the plant samples being treated with target compounds. The results show that the compounds Z3, Z5, and Z10 target AtPYL2 and that the dissociation constants for binding by MST were 3.59, 3.54, and 3.97 µmol/L, respectively, among them, and the molecular docking results showed that compounds Z3, Z5, and Z10 formed hydrophobic interactions with amino acid residues through hydrogen or halogen bonding. This highlights their potential as an ABA receptor protein agonist. On the other hand, in vivo, compounds Z3, Z5, and Z10 had different inhibitory effects on seed germination, with compound Z5 inhibiting the root growth of A. thaliana and compound Z10 affecting root growth. In conclusion, these compounds could regulate plant growth and could be further developed as new plant-regulating agents.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / Arabidopsis Proteins Language: En Journal: J Agric Food Chem Year: 2023 Document type: Article Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / Arabidopsis Proteins Language: En Journal: J Agric Food Chem Year: 2023 Document type: Article Country of publication: United States