SlbHLH152, a bHLH transcription factor positively regulates iron homeostasis in tomato.
Plant Sci
; 335: 111821, 2023 Oct.
Article
in En
| MEDLINE
| ID: mdl-37558055
ABSTRACT
The maintain of iron (Fe) homeostasis is essential for plant survival. In tomato, few transcription factors have been identified as regulators of Fe homeostasis, among which SlbHLH068 induced by iron deficiency, plays an important role. However, the upstream regulator(s) responsible for activating the expression of SlbHLH068 remain(s) unknown. In this study, the bHLH (basic helix-loop-helix) transcription factor SlbHLH152 was identified as an upstream regulator of SlbHLH068 using yeast one-hybrid screening. Deletion of SlbHLH152 led to a significant decline in Fe concentration, which was accompanied by reduced expression of Fe-deficiency-responsive genes. In contrast, SlbHLH152 overexpression plants displayed tolerance to iron deficiency, increased Fe accumulation, and elevated expression of Fe-deficiency-responsive genes. Further analysis indicated that SlbHLH152 directly activates the transcription of SlbHLH068. Taken together, our results suggest that SlbHLH152 may be involved in the regulation of iron homeostasis by directly activating the transcription of SlbHLH068 in tomato.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Solanum lycopersicum
/
Arabidopsis Proteins
/
Iron Deficiencies
Type of study:
Prognostic_studies
Language:
En
Journal:
Plant Sci
Year:
2023
Document type:
Article
Affiliation country:
China
Publication country:
IE
/
IRELAND
/
IRLANDA