High-throughput differential scanning fluorimetry (DSF) and cellular thermal shift assays (CETSA): Shifting from manual to automated screening.
SLAS Technol
; 28(6): 411-415, 2023 12.
Article
in En
| MEDLINE
| ID: mdl-37598756
ABSTRACT
Biophysical affinity screening is increasingly being adopted as a high-throughput hit finding technique in drug discovery. Automation is highly beneficial to high-throughput screening (HTS) since a large number of compounds need to be reproducibly tested against a biological target. Herein, we describe how we have automated two biophysical affinity screening methods that rely on a thermal shift in protein melting temperature upon small molecule binding differential scanning fluorimetry (DSF) and the cellular thermal shift assay (CETSA).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Drug Discovery
/
High-Throughput Screening Assays
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
En
Journal:
SLAS Technol
Year:
2023
Document type:
Article