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ReACT (redox-activated chemical tagging) chemistry enables direct derivatization and fluorescence detection of S-adenosyl-L-homocysteine (SAH).
Lin, Bohong; Xiang, Lingling; Yuan, Zhijun; Hou, Qi; Ruan, Yaoping; Zhang, Jing.
Affiliation
  • Lin B; Artemisinin Research Center and The First Affiliated Hospital, Guangzhou University of Chinese Medicine, 12 Jichang Road, Guangzhou 510405, China. jingzhang@gzucm.edu.cn.
  • Xiang L; Artemisinin Research Center and The First Affiliated Hospital, Guangzhou University of Chinese Medicine, 12 Jichang Road, Guangzhou 510405, China. jingzhang@gzucm.edu.cn.
  • Yuan Z; Artemisinin Research Center and The First Affiliated Hospital, Guangzhou University of Chinese Medicine, 12 Jichang Road, Guangzhou 510405, China. jingzhang@gzucm.edu.cn.
  • Hou Q; Artemisinin Research Center and The First Affiliated Hospital, Guangzhou University of Chinese Medicine, 12 Jichang Road, Guangzhou 510405, China. jingzhang@gzucm.edu.cn.
  • Ruan Y; Artemisinin Research Center and The First Affiliated Hospital, Guangzhou University of Chinese Medicine, 12 Jichang Road, Guangzhou 510405, China. jingzhang@gzucm.edu.cn.
  • Zhang J; Artemisinin Research Center and The First Affiliated Hospital, Guangzhou University of Chinese Medicine, 12 Jichang Road, Guangzhou 510405, China. jingzhang@gzucm.edu.cn.
Org Biomol Chem ; 21(35): 7085-7089, 2023 09 13.
Article in En | MEDLINE | ID: mdl-37602780
ABSTRACT
S-Adenosyl-L-homocysteine (SAH) is a universal byproduct and product inhibitor of the methyltransferase-catalyzed methylation reaction. Here based on ReACT (redox-activated chemical tagging) chemistry, direct derivatization and fluorescence measurement of SAH were achieved with features such as mild reaction conditions and simple operation.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: S-Adenosylhomocysteine / Homocysteine Type of study: Diagnostic_studies Language: En Journal: Org Biomol Chem Journal subject: BIOQUIMICA / QUIMICA Year: 2023 Document type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: S-Adenosylhomocysteine / Homocysteine Type of study: Diagnostic_studies Language: En Journal: Org Biomol Chem Journal subject: BIOQUIMICA / QUIMICA Year: 2023 Document type: Article Affiliation country: China