Simple chitin-based cell culture platform for production of biopharmaceuticals.
Biotechnol Lett
; 45(10): 1265-1277, 2023 Oct.
Article
in En
| MEDLINE
| ID: mdl-37606752
ABSTRACT
OBJECTIVES:
Gene therapy using viral vectors and antibody-based therapies continue to expand within the pharmaceutical market. We evaluated whether Cellhesion® VP, a chitin-based material, can be used as 3D culture platform for cell lines used for the production of antibodies and viral vectors.RESULTS:
The results of Cell Counting Kit-8 assay and LDH assay revealed that Cellhesion® VP had no adverse effect to Human Embryonic Kidney (HEK) 293, A549 and Chinese hamster ovary (CHO) DG44-Interferon-ß (IFN) cells. Cell growth analyses showed that Cellhesion® VP supported the 3D culture of HEK293, A549 and CHO DG44- IFN-ß cells with a spherical morphology. Importantly, subculture of these cell lines on Cellhesion® VP was easily performed without trypsinization because cells readily transferred to newly added scaffold. Our data also suggest that CHO DG44-IFNß, cultured on Cellhesion® VP secreted IFNß stably and continuously during the culture period.CONCLUSIONS:
Cellhesion® VP provides a simple and streamlined expansion culture system for the production of biopharmaceuticals.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biological Products
Limits:
Animals
/
Humans
Language:
En
Journal:
Biotechnol Lett
Year:
2023
Document type:
Article
Affiliation country:
Japan