Dual RNA-seq identifies genes and pathways modulated during Clostridioides difficile colonization.
mSystems
; 8(5): e0055523, 2023 Oct 26.
Article
in En
| MEDLINE
| ID: mdl-37615437
ABSTRACT
IMPORTANCE The initial interactions between the colonic epithelium and the bacterium are likely critical in the establishment of Clostridioides difficile infection, one of the major causes of hospital-acquired diarrhea worldwide. Molecular interactions between C. difficile and human gut cells have not been well defined mainly due to the technical challenges of studying cellular host-pathogen interactions with this anaerobe. Here we have examined transcriptional changes occurring in the pathogen and host cells during the initial 24 hours of infection. Our data indicate several changes in metabolic pathways and virulence-associated factors during the initial bacterium-host cell contact and early stages of infection. We describe canonical pathways enriched based on the expression profiles of a dual RNA sequencing in the host and bacterium, and functions of bacterial factors that are modulated during infection. This study thus provides fresh insight into the early C. difficile infection process.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Clostridioides difficile
/
Clostridium Infections
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
MSystems
Year:
2023
Document type:
Article
Affiliation country:
United kingdom