Adverse outcome pathway-based analysis of liver steatosis in vitro using human liver cell lines.
STAR Protoc
; 4(3): 102500, 2023 Sep 15.
Article
in En
| MEDLINE
| ID: mdl-37616165
ABSTRACT
Here, we present an in vitro test battery to analyze chemicals for their potential to induce liver triglyceride accumulation, a hallmark of liver steatosis. We describe steps for using HepG2 and HepaRG human hepatoma cells in conjunction with a combination of several in vitro assays covering the different molecular initiating events and key events of the respective adverse outcome pathway. This protocol is suitable for assessing single substance effects as well as mixtures allowing their classification as steatotic or non-steatotic. For complete details on the use and execution of this protocol, please refer to Luckert et al. (2018),1 Lichtenstein et al. (2020),2 and Knebel et al. (2019).3.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carcinoma, Hepatocellular
/
Fatty Liver
/
Adverse Outcome Pathways
Limits:
Humans
Language:
En
Journal:
STAR Protoc
Year:
2023
Document type:
Article
Affiliation country:
Germany