An mTRAN-mRNA interaction mediates mitochondrial translation initiation in plants.
Science
; 381(6661): eadg0995, 2023 09.
Article
in En
| MEDLINE
| ID: mdl-37651534
ABSTRACT
Plant mitochondria represent the largest group of respiring organelles on the planet. Plant mitochondrial messenger RNAs (mRNAs) lack Shine-Dalgarno-like ribosome-binding sites, so it is unknown how plant mitoribosomes recognize mRNA. We show that "mitochondrial translation factors" mTRAN1 and mTRAN2 are land plant-specific proteins, required for normal mitochondrial respiration chain biogenesis. Our studies suggest that mTRANs are noncanonical pentatricopeptide repeat (PPR)-like RNA binding proteins of the mitoribosomal "small" subunit. We identified conserved Adenosine (A)/Uridine (U)-rich motifs in the 5' regions of plant mitochondrial mRNAs. mTRAN1 binds this motif, suggesting that it is a mitoribosome homing factor to identify mRNAs. We demonstrate that mTRANs are likely required for translation of all plant mitochondrial mRNAs. Plant mitochondrial translation initiation thus appears to use a protein-mRNA interaction that is divergent from bacteria or mammalian mitochondria.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptide Chain Initiation, Translational
/
Plant Proteins
/
RNA, Messenger
/
Mitochondria
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Science
Year:
2023
Document type:
Article
Affiliation country:
Sweden