CD100 boosts the inflammatory response in the challenge phase of allergic contact dermatitis in mice.
Contact Dermatitis
; 89(6): 442-452, 2023 Dec.
Article
in En
| MEDLINE
| ID: mdl-37700557
ABSTRACT
BACKGROUND:
Allergic contact dermatitis (ACD) is an inflammatory disease with a complex pathophysiology in which epidermal-resident memory CD8+ T (TRM ) cells play a key role. The mechanisms involved in the activation of CD8+ TRM cells during allergic flare-up responses are not understood.METHODS:
The expression of CD100 and its ligand Plexin B2 on CD8+ TRM cells and keratinocytes before and after allergen exposure was determined by flow cytometry and RT-qPCR. The role of CD100 in the inflammatory response during the challenge phase of ACD was determined in a model of ACD in CD100 knockout and wild-type mice.RESULTS:
We show that CD8+ TRM cells express CD100 during homeostatic conditions and up-regulate it following re-exposure of allergen-experienced skin to the experimental contact allergen 1-fluoro-2,4-dinitrobenzene (DNFB). Furthermore, Plexin B2 is up-regulated on keratinocytes following exposure to some contact allergens. We show that loss of CD100 results in a reduced inflammatory response to DNFB with impaired production of IFNγ, IL-17A, CXCL1, CXCL2, CXCL5, and IL-1ß and decreased recruitment of neutrophils to the epidermis.CONCLUSION:
Our study demonstrates that CD100 is expressed on CD8+ TRM cells and is required for full activation of CD8+ TRM cells and the flare-up response of ACD.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dermatitis, Allergic Contact
Limits:
Animals
Language:
En
Journal:
Contact Dermatitis
Year:
2023
Document type:
Article
Affiliation country:
Denmark